Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1–2 by Directed Evolution
نویسندگان
چکیده
منابع مشابه
Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1-2 by Directed Evolution.
The glycoside hydrolase LXYL-P1-2 from Lentinula edodes can specifically hydrolyze 7-β-xylosyltaxanes to form 7-β-hydroxyltaxanes for the semi-synthesis of paclitaxel. In order to improve the catalytic properties of the enzyme, we performed error-prone PCR to construct the random mutant library of LXYL-P1-2 and used the methanol-induced plate method to screen the mutants with improved catalytic...
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ژورنال
عنوان ژورنال: Molecules
سال: 2017
ISSN: 1420-3049
DOI: 10.3390/molecules22122133